Thoughts on Thawing

Thawing frozen samples correctly is essential for accurate testing. Follow these straightforward steps to ensure your results are reliable.

The Best Way: Using a Circulating Water Bath

• Set the Temperature: Use a water bath set to 37°C.
• Thawing Time: For small vials (1.0 mL), it usually takes 3–5 minutes. Larger samples may will need extra time (ask your account representative for instructions on thawing bulk containers).
• Check Progress: Make sure the sample is completely thawed but avoid leaving it in the water bath for too long.

Water baths are ideal because they:

• Thaw samples quickly and evenly.
• Provide steady heat without fluctuations, even with multiple samples.
• Reduce the risk of overheating compared to heat blocks.

Final Tips

• Always stick to 37°C when thawing.
• Mix the sample gently and thoroughly after thawing to ensure consistency.
• Avoid overheating, as it can damage the sample.

By following these simple steps, you can maintain the quality of your samples and get accurate results every time!

A – Always store at -70°C or colder (can store at -20°C for use within 1 week).

B – Be sure to thaw samples for 3-5 minutes in a 37°C circulating water bath.

C – Coagulation Testing – For our CORE and SysCOR Kits, it is best to thaw 4-5 samples at a time, mix gently, place samples on analyzer, and repeat process until all samples are tested.